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JHC1-64 binds specifically to SERT. Confocal microscopy images of co-localization between EGFP-SERT were transiently expressed in CAD and the fluorescent cocaine analog JHC1-64. Images were taken after 10 min of incubation with 10 nm JHC1-64 alone or together with 1 μm of the SERT-specific antagonist paroxetine. Cells treated with paroxetine were preincubated for 10 min before the addition of JHC1-64. Data are representative of three independent experiments. Scale bar, 20 μm.
