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Positive charge in the γNT is required for stability and secondary structure in the presence of PIP2. Mutation of γNT K-cluster to glutamines does not show significant difference in stability or secondary structure in the presence or absence of PIP2 (A), whereas mutation to arginines increases stability and secondary structure content in the presence of PIP2 compared with in its absence (B) (compare with Fig. 1). SS, secondary structure.
