FIGURE 2.

Characterization of Xenopus TDG antibody. TDG antiserum (left) or the matched preimmune serum (right) was used for Western blotting to probe 0.6 ng of recombinant TDG (lanes 1 and 4), 0.6 μl of a 2:1 mixture of NPE (diluted by 50% with ELB) plus HSS (lanes 2 and 5), and 0.6 ng of TDG combined with 0.6 μl of the NPE/HSS mixture (lanes 3 and 6). The band running at 50 kDa is a nonspecific cross-reacting band, as evidenced by the fact that it is not recognized by antiserum from a second rabbit that efficiently recognizes TDG.