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Effect of PKI-GFP or RevAB-GFP expression on β-agonist-mediated cAMP accumulation. GFP-, PKI-GFP-, or RevAB-GFP-expressing HASM cells were plated into 24-well plates and stimulated with vehicle (Bas), ISO (50 nm or 1 μm), or FSK (100 μm). Global cAMP accumulation was measured after 10-min stimulation and plotted as raw values (picomoles/well) (A) or percent of the FSK-stimulated value (B). Data are mean ± S.E. *, p < 0.05 PKI/RevAB versus GFP (n = 6).
