FIGURE 7.

Effect of Epac knockdown on β-agonist regulation of VASP, phospho-HSP-20, phospho-p42/p44, and phospho-MLC-20 levels. 96 h after transfection of HASM cells with either scrambled (Scr) siRNA or siRNA targeting Epac1, cells plated into 12-well plates were stimulated with vehicle (Bas), ISO (1 μm), 8-pCPT-2′-O-Me-cAMP (8CPT, 100 μm), or FSK (100 μm) ± HIST (1 μm) as described under “Experimental Procedures.” After 5 min of stimulation, cell lysates were harvested, and expression levels of VASP, phospho-HSP-20, phospho-p42/p44 and phospho-MLC-20, and β-actin were assessed by immunoblotting. Blots are representative of three experiments providing similar results.