FIGURE 4.

B-Raf knockdown reduces adhesion to VCAM-1 and fibronectin. A, Western blot using SDS-PAGE (10% gel) of whole cell lysates (1 × 10⁷ cells) of Jurkat, vector control (VC), and B-Raf KD cells cultured with and without doxycycline (±D), and probed for total B-Raf or β-actin. B and C, parallel plate flow detachment assay of vector control and KD cells cultured with doxycycline (+D) or without doxycycline (NT) to slides coated with human VCAM-1 (10 μg/ml) (B) or human FN (5 μg/ml) (C). D and E, percentage of cells remaining at maximum shear from the parallel plate flow assay of vector control and KD cells to slides coated with VCAM-1 (D) or FN (E). Error bars, S.E. *, p < 0.01 using Student's t test.