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. 2014 Jun 17;289(33):23154–23167. doi: 10.1074/jbc.M114.570812

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Stress induces H4K5ac/K8ac deacetylation and H3S10ph dephosphorylation. A, outline for the MONF protocol. B, effect of stress treatment on the histone modifications of nucleosomal H3 and H4. NLF was prepared from HeLa cells with the indicated treatment and analyzed for the indicated modifications by Western blot (WB). C, ChIP-qPCR analysis of nucleosomal H3S10ph, H4K5ac, and H4K8ac levels in promoter and gene body regions of the indicated genes after HMBA treatments of HIV-LTR-luciferase cells. The levels in non-treated cells were set as 100%. D, flow cytometry analysis of cell cycle distribution after indicated stress treatments of non-synchronized HeLa cells. E, effect of stress on H3S10ph and H4K5ac/K8ac in nocodazole-synchronized HeLa cells. F, effect of stress on H3S10ph and H4K5ac/K8ac in serum-starved HCT116 cells.