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. 2014 Jun 25;289(33):23219–23232. doi: 10.1074/jbc.M114.577205

FIGURE 5.

FIGURE 5.

Removal of the GroEL C termini diminishes both forced unfolding and compaction of Rubisco. a and b, change in FRET efficiency of labeled Rubisco (100 nm), bound to the trans ring of either a full-length GroEL-GroES (blue) or ELΔ526-GroES (green) complex (120 nm), during encapsulation by GroES. The change in FRET efficiency for 34ED-454F is shown in a and for 209ED-58F is shown in b. c and d, change in FRET efficiency of labeled Rubisco (100 nm) bound to the single ring GroEL variants SR1 (blue) or SRΔ526 (green) (500 nm), during encapsulation by GroES (1 μm). The change in FRET efficiency for 34ED-454F labeled Rubisco is shown in c and 209ED-58F is shown in d. In each case, the starting FRET efficiency of non-native Rubisco bound to each GroEL variant is shown, beginning at t= 0. At t = 2.25 s (arrow), the complex was rapidly mixed with ATP and GroES; n = 21 replicates, with sampling times of 20 ms. The small gap in each plot is a consequence of the removal of data points collected during the mixing dead time.