TABLE 1.
Intramolecular FRET measurements of Rubisco bound to wild-type and Δ526 GroEL
| FRET pair | Distancea |
||
|---|---|---|---|
| WTb | Δ526c | ||
| Å | |||
| Steady state | 34EDd-454Fe | 44.1 | 42.0 |
| 58F-209ED | 46.9 | 41.2 | |
| 58F-356ED | 43.9 | 40.1 | |
| 58F-454ED | 46.2 | 42.0 | |
| Time-resolved | 34ED-454F | 46.1 | 43.5 |
| 58F-209ED | 49.1 | 41.7 | |
| 58F-356ED | 43.2 | 39.3 | |
| 58F-454ED | 44.5 | 42.3 | |
a Steady-state and time-resolved intramolecular FRET measurements between different positions on chemically denatured, doubly-labeled RuBisCO bound to the trans ring of Δ526 GroEL/GroES ADP bullets.
b Full-length GroEL/GroES ADP bullets.
c Δ526 GroEL/GroES ADP bullets.
d Rubisco labeled with EDANS at amino acid 34.
e Rubisco labeled with fluorescein at amino acid 454.