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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Microsc. 2013 Nov 19;253(2):83–92. doi: 10.1111/jmi.12099

Figure 1.

Figure 1

The parabolic mirror assembly surrounds an objective on a regular commercially available upright two-photon microscope. Emission light collected by the parabola and the objective is reflected from a dichroic mirror in the turret and sent through an emission filter and focusing lens onto a wide-are PMT. The inset picture shows the relationship of the parabola to the sample for an experiment involving imaging of a leg muscle (Tibialis anterior). The dichroic mirror can be moved by a slider to enable standard wide-field imaging. Also shown is the thread system that allows focusing of the parabola.