Confocal fluorescence images of A549
cells. A549 cells were treated
with different concentrations of Au-SiNW nanohybrids and NIR light
centered at 808 nm (0.30 W/cm2) for 20 min. The concentration
of Au-SiNW nanohybrids was 0.100 mg/mL without NIR irradiation (a)
and 0.100 (b), 0.050 (c), 0.025 (d), 0.010 (e), and 0.0 mg/mL (f)
with NIR irradiation. After staining a cell population, apoptotic
cells showed green fluorescence stained by YO-PRO-1, necrotic cells
showed red color by PI, and control cells showed little or no fluorescence.
DIC = differential interference contrast. Scale bar = 100 μm.