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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Leuk Res. 2009 Aug 12;34(2):173–176. doi: 10.1016/j.leukres.2009.07.030

Figure 1.

Figure 1

Serum starvation induces down-regulation of Ki-67 protein. A) K562, HL60, and Raji cells were cultured in media containing 10% FBS (■; Control) or 0.2% FBS (□; Serum Starvation). Lysates were prepared from the cells indicated, and Ki-67 levels were evaluated using the Meso Scale Discovery (MSD)-based enzyme immunoassay. Data represent the mean (SD) of at least 3 experiments. B) Lysates (100 μg) from cells cultured in media containing 10% (Control) or 0.2% (Serum Starvation) FBS were analyzed by SDS-PAGE/immunoblotting using anti-Ki-67, anti-PCNA or anti-actin antibodies. Antibody detection was accomplished by electrochemiluminescence with exposure to x-ray film. All experiments were repeated at least 3 times.