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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Leuk Res. 2009 Aug 12;34(2):173–176. doi: 10.1016/j.leukres.2009.07.030

Figure 2.

Figure 2

Detection of circulating Ki-67 protein in the plasma of patients with acute lymphoblastic leukemia (ALL), but not control subjects, by immunoblot assay. K562 extracts (100 μg, lane 1) and diluted plasma from 4 normal control subjects and 4 ALL patients (1:5 in lysis buffer, lanes 2-9) were analyzed by SDS-PAGE/immunoblotting using anti-albumin (bottom) antibodies. Alternatively, lysate and plasma were prepared and subjected to immunoprecipitation (IP) with anti-Ki-67 antibody or anti-IgG antibody (top and middle). The resulting immune complexes were analyzed by Western blotting using Ki-67 antibody, as indicated. Antibody detection was accomplished by enhanced chemiluminescence with exposure to x-ray film.