Skip to main content
. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Free Radic Biol Med. 2010 Sep 7;49(11):1694–1702. doi: 10.1016/j.freeradbiomed.2010.08.029

Fig. 4.

Fig. 4

mRNA levels of Gpx1 and Gpx3 in the ileum (A), proximal (B) and distal (C) colon of wild-type and GPx2 KO mice at different selenium status.

RNA was isolated and GPx1 and 3 mRNA quantified in triplicate by PCR and normalized to the reference gene Rpl13a (see Methods). Selenium state and genotype is indicated in the figure. Levels are expressed relative to the level of selenium-restricted wild-type mice, which was set to 1. Values are means ± SD (n = 4). Significance was estimated by two way ANOVA with Bonferroni's post tests. #p<0.05, ##p<0.01, ###p<0.001 vs. respective groups of Se-restricted (-Se) mice. For further details see ‘Methods’.