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. 2014 Aug 9;14:580. doi: 10.1186/1471-2407-14-580

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© Horie et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Knockdown of TGF-β ligands. A: TGF-β1 and TGF-β2 concentrations measured by ELISA in the supernatant of A549 cells transduced with each miRNA. Left: TGF-β1. Right: TGF-β2. Data shown are the means ± SEM of triplicate analyses. KD: knockdown. NTC: negative control. The concentration of TGF-β1 or TGF-β2 in the supernatant of cells with TGF-β1 and/or TGF-β2 knockdown was compared to that of cells transduced with NTC miRNA. Statistical significance was determined by Dunnett’s test. ^* P < 0.05. B: TGF-β1 and TGF-β2 concentrations measured by ELISA in the supernatant of HFL-1 cells.