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. 2014 Aug 9;14:580. doi: 10.1186/1471-2407-14-580

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© Horie et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cell proliferation assay. Cell proliferation curve in A549 or HFL-1 cells transduced with NTC miRNA (solid line) compared to cells transduced with miRNA against TGF-β1 (dashed line: TGF-β1 KD), TGF-β2 (dotted line: TGF-β2 KD), or TGF-β1 and TGF-β2 (dashed-dotted line: TGF-β1 + β2 KD). Cell counts were carried out on days 1, 3, and 5 after seeding. Left: A549. Right: HFL-1. Data shown are the means ± SEM of triplicate analyses. Numbers of cells with TGF-β1 and/or TGF-β2 knockdown on day 5 was compared to that in the cells transduced with NTC miRNA. Statistical significance was determined by Student’s t-test. ^* P < 0.05.