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. 2014 Aug 9;14:580. doi: 10.1186/1471-2407-14-580

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© Horie et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Collagen gel contraction assay. Time-course of gel contraction in the presence of HFL-1 transduced with NTC miRNA (solid line), or miRNAs against TGF-β1 (dashed line: TGF-β1 KD), TGF-β2 (dotted line: TGF-β2 KD), or TGF-β1 and TGF-β2 (dashed-dotted line: TGF-β1 + β2 KD). The area of each gel was assessed daily for 5 days and the relative value compared to the initial size was determined. Data shown are the means ± SD of triplicate analyses. Statistical significance was determined by Student’s t-test. *P < 0.05.