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. 2014 Jul 25;15(1):628. doi: 10.1186/1471-2164-15-628

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© Yang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The polyhedrin protein matrix and occlusion bodies (OBs) production of recombinant AcMNPV- polh (PmNV) and wild-type AcMNPV in Sf9 at 3 days post-infection. (A) Photomicrographs of Sf9 cells infected with recombinant AcMNPV-polh (PmNV), wild-type AcMNPV, recombinant AcMNPV-vp28 (WSSV) as non-occluded control and non-infected cells (Normal cells). Examples of cells producing polyhedrin protein matrix and OBs are indicated by arrows. (B) Photomicrographs of purified polyhedrin protein matrix and AcMNPV OBs. Scale bars are 20 μm.