Table 4.
Annotation of PmNV putative ORFs
| ORF | Strand | Position | Length | Best BLAST match | Signature | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Start | End | nt | aa | ORF, protein encoded, or mass | Species | aa identity (% match identity) | E value | |||
| 1 | + | 1 | 1359 | 1356 | 452 | polyhedrin | ||||
| 2 | - | 1525 | 1989 | 462 | 154 | coiled-coil | ||||
| 3 | - | 2033 | 4861 | 2826 | 942 | von Willebrand factor type A | Frankia sp. CN3 | 33 | 8 × 10-3 | vWFA, ZF_RING_2 |
| 4 | - | 4954 | 5913 | 957 | 319 | |||||
| 5 | + | 5998 | 9273 | 3273 | 1091 | DNA polymerase | Helicoverpa zea nudivirus 2 | 26 | 2 × 10-102 | POLBc |
| 6 | + | 9354 | 10559 | 1203 | 401 | methyltransferase | Ceratitis capitata | 33 | FtsJ | |
| 7 | - | 10563 | 10772 | 207 | 69 | TM | ||||
| 8 | - | 10779 | 11455 | 684 | 228 | Ac92-like (sulfhydryl oxidase) | Heliothis zea virus 1 | 42 | 1 × 10-17 | Evr1_Alr, TM |
| 9 | - | 11455 | 13482 | 2025 | 675 | vp91 (capsid) | Oryctes rhinoceros virus | 31 | 7 × 10-32 | ChtBD2, TM |
| 10 | + | 13622 | 14971 | 1347 | 449 | Odv-e56/pif-5 (envelope) | Heliothis zea virus 1 | 27 | 2 × 10-30 | TM |
| 11 | - | 13780 | 14331 | 549 | 183 | TM | ||||
| 12 | + | 15057 | 15536 | 477 | 159 | |||||
| 13 | - | 15325 | 15549 | 222 | 74 | TM | ||||
| 14 | + | 15548 | 16807 | 1257 | 419 | p47 | Heliothis zea virus 1 | 24 | 1 × 10-5 | |
| 15 | - | 16816 | 18108 | 1290 | 430 | per-os infectivity factor 2 (pif-2) | Macrobrachium nudivirus | 66 | 2 × 10-75 | Baculo_44, coiled-coil, TM |
| 16 | + | 17191 | 17598 | 405 | 135 | OHCU decarboxylase | Sarcophilus harrisii | 27 | 3 × 10-3 | TM |
| 17 | - | 18139 | 18987 | 846 | 282 | HZV_115-like | Oryctes rhinoceros virus | 33 | 0.58 | p-loop_NTPase, NK superfamily |
| 18 | - | 19071 | 20372 | 1299 | 433 | |||||
| 19 | + | 20465 | 20869 | 402 | 134 | |||||
| 20 | + | 20812 | 22083 | 1269 | 423 | flap endonuclease 1 (FEN-1) | Heliothis zea virus 1 | 29 | 3 × 10-14 | |
| 21 | + | 22080 | 22556 | 474 | 158 | |||||
| 22 | - | 22553 | 23485 | 930 | 310 | 31 K structural protein | Chelonus inanitus | 24 | 1 × 10-9 | |
| 23 | + | 23615 | 26713 | 3096 | 1032 | RNA polymerase (lef-8) | Heliothis zea virus 1 | 28 | 8 × 10-53 | RNA_pol_Rpb2_6 |
| 24 | + | 26864 | 28057 | 1191 | 397 | p51 late protein | Heliothis zea virus 1 | 23 | 4 × 10-9 | SBP_BACTERIAL_1 |
| 25 | - | 28160 | 31906 | 3744 | 1248 | AF-4, ZnF_C2H2 | ||||
| 26 | + | 31688 | 31912 | 222 | 74 | TM | ||||
| 27 | - | 31969 | 32715 | 744 | 248 | |||||
| 28 | - | 34005 | 34214 | 210 | 69 | |||||
| 29 | - | 34463 | 42040 | 7572 | 2524 | TM | ||||
| 30 | - | 42077 | 42649 | 570 | 190 | |||||
| 31 | + | 43013 | 43204 | 189 | 63 | |||||
| 32 | - | 43300 | 43512 | 210 | 70 | |||||
| 33 | + | 43884 | 44783 | 897 | 299 | tripartite motif-containing protein 10-like | Alligator mississippiensis | 37 | 7 × 10-9 | RING, coiled-coil |
| 34 | - | 44939 | 46594 | 1653 | 551 | odv-e66 (chondroitin lyase) | Oryctes rhinoceros virus | 29 | 1 × 10-23 | odv-e66 |
| 35 | + | 47292 | 48599 | 1305 | 435 | |||||
| 36 | - | 48994 | 50901 | 1905 | 635 | odv-e66 (chondroitin lyase) | Oryctes rhinoceros virus | 26 | 5 × 10-22 | odv-e66, TM |
| 37 | - | 51077 | 51280 | 201 | 67 | |||||
| 38 | + | 51353 | 52297 | 942 | 314 | Guanosine monophosphate kinase | Heliothis zea virus 1 | 31 | 5 × 10-17 | |
| 39 | + | 52359 | 53936 | 1575 | 525 | per-os infectivity factor 1 (pif-1) | Heliothis zea virus 1 | 35 | 5 × 10-57 | PIF, TM |
| 40 | - | 53953 | 54573 | 618 | 206 | |||||
| 41 | + | 54167 | 54595 | 426 | 142 | TM | ||||
| 42 | - | 54573 | 56423 | 1848 | 616 | orf21 | Helicoverpa zea nudivirus 2 | 25 | 1 × 10-7 | |
| 43 | + | 56796 | 57572 | 774 | 258 | |||||
| 44 | + | 57636 | 58376 | 738 | 246 | |||||
| 45 | + | 58384 | 59130 | 744 | 248 | histidine decarboxylase (PmV-like) | Paramecium bursaria Chlorella virus NY-2B | 27 | 0.15 | |
| 46 | + | 59185 | 60201 | 1014 | 338 | thymidylate kinase | Heliothis zea virus 1 | 27 | 2 × 10-14 | p-loop_NTPase |
| 47 | + | 60297 | 61211 | 912 | 304 | |||||
| 48 | + | 61235 | 63007 | 1770 | 590 | DNA ligase-like | Helicoverpa zea nudivirus 2 | 24 | 2 × 10-6 | |
| 49 | + | 63022 | 63729 | 705 | 235 | SJCHGC06586 protein | Schistosoma japonicum | 38 | 1 × 10-7 | RING, coiled-coil |
| 50 | - | 63726 | 64037 | 309 | 103 | |||||
| 51 | + | 64012 | 64221 | 207 | 69 | TM | ||||
| 52 | + | 64249 | 64776 | 525 | 175 | late expression factor 5 (lef-5) | Spodoptera frugiperda MNPV | 31 | 0.028 | Baculo_LEF-5_C |
| 53 | + | 65083 | 65247 | 162 | 54 | orf9 | Helicoverpa zea nudivirus 2 | 37 | 0.003 | TM |
| 54 | + | 65268 | 65930 | 660 | 220 | |||||
| 55 | + | 65979 | 66899 | 918 | 306 | integrase | Helicoverpa zea nudivirus 2 | 33 | 1 × 10-32 | INT_REC_C |
| 56 | + | 66883 | 67752 | 867 | 289 | very late expression factor 1 | Heliothis zea virus 1 | 32 | 5 × 10-11 | DNA_REC_C |
| 57 | - | 67753 | 67959 | 204 | 68 | coiled-coil | ||||
| 58 | - | 67965 | 69689 | 1722 | 574 | lef-9 | Heliothis zea virus1 | 32 | 3 × 10-63 | |
| 59 | + | 69705 | 70553 | 846 | 282 | 38 K protein | Chelonus inanitus | 37 | 2 × 10-17 | |
| 60 | - | 70543 | 70824 | 279 | 93 | |||||
| 61 | + | 70823 | 71515 | 690 | 230 | orf49 | Helicoverpa zea nudivirus 2 | 29 | 2.9 | |
| 62 | + | 71512 | 71862 | 348 | 116 | TM | ||||
| 63 | - | 71859 | 72215 | 354 | 118 | |||||
| 64 | - | 72286 | 72633 | 345 | 115 | TM | ||||
| 65 | - | 72717 | 74030 | 1311 | 437 | orf51 | Heliothis zea virus1 | 30 | 7 × 10-30 | p-loop_NTPase |
| 66 | + | 74079 | 75329 | 1248 | 416 | orf84 | Helicoverpa zea nudivirus 2 | 23 | 9 × 10-4 | |
| 67 | - | 75597 | 76169 | 573 | 191 | Crocosphaera watsonii | 25 | 1 × 10-4 | ||
| 68 | - | 76400 | 76963 | 561 | 187 | |||||
| 69 | - | 76980 | 77966 | 984 | 328 | HzNVORF106-like protein | Cotesia congregata | 24 | 1 × 10-6 | |
| 70 | - | 77935 | 78555 | 618 | 206 | |||||
| 71 | - | 78633 | 80174 | 1539 | 513 | |||||
| 72 | - | 80247 | 82301 | 2052 | 684 | p74 / pif-0 (envelope) | Heliothis zea virus 1 | 30 | 4 × 10-95 | Baculo_74, TM |
| 73 | + | 82393 | 83277 | 882 | 294 | Zonadhesin | Sorex araneus | 49 | 2 × 10-52 | TM |
| 74 | - | 82986 | 83258 | 237 | 90 | TM | ||||
| 75 | + | 83362 | 83934 | 570 | 190 | |||||
| 76 | + | 84006 | 85736 | 1728 | 576 | helicase 2 | Danaus plexippus | 30 | 1 × 10-46 | |
| 77 | + | 85788 | 86687 | 897 | 299 | |||||
| 78 | - | 86652 | 87311 | 657 | 219 | orf100 | Heliothis zea virus 1 | 23 | 0.014 | |
| 79 | + | 87109 | 89256 | 2145 | 715 | helicase 2 | Danaus plexippus | 28 | 5 × 10-39 | TM |
| 80 | + | 89240 | 92104 | 2862 | 954 | coiled-coil | ||||
| 81 | - | 90949 | 91242 | 291 | 97 | |||||
| 82 | + | 92108 | 93043 | 933 | 311 | |||||
| 83 | + | 93036 | 93647 | 609 | 203 | |||||
| 84 | - | 93644 | 95206 | 1560 | 520 | |||||
| 85 | - | 95208 | 95618 | 408 | 136 | |||||
| 86 | + | 95617 | 96090 | 471 | 157 | Ac81-like | Helicoverpa zea nudivirus 2 | 36 | 3 × 10-21 | TM |
| 87 | + | 96083 | 97993 | 1908 | 636 | |||||
| 88 | + | 98006 | 98437 | 429 | 143 | Ac68-like/pif-6 | Helicoverpa zea nudivirus 2 | 29 | 8 × 10-11 | TM |
| 89 | - | 98438 | 99691 | 1251 | 417 | |||||
| 90 | + | 99781 | 100530 | 720 | 240 | VLF-1 protein | Helicoverpa zea nudivirus 2 | 28 | 5 × 10-5 | |
| 91 | - | 100500 | 101912 | 1410 | 470 | late expression factor 4 | Helicoverpa zea nudivirus 2 | 26 | 4 × 10-18 | |
| 92 | - | 101932 | 102255 | 321 | 107 | |||||
| 93 | - | 102312 | 102890 | 576 | 192 | per-os infectivity factor 3 | Helicoverpa zea nudivirus 2 | 38 | 4 × 10-26 | TM |
| 94 | - | 102887 | 106702 | 3813 | 1271 | helicase | Helicoverpa zea nudivirus 2 | 28 | 3 × 10-19 | primase_Cte |
| 95 | + | 106149 | 106370 | 219 | 73 | |||||
| 96 | + | 106701 | 107399 | 696 | 232 | 19kda protein/Odv-e28/pif-4 | Oryctes rhinoceros virus | 38 | 5 × 10-21 | Baculo_19, TM |
| 97 | - | 107374 | 108123 | 747 | 249 | |||||
| 98 | + | 108113 | 108946 | 831 | 277 | Esterase/lipase | Helicoverpa zea nudivirus 2 | 27 | 3 × 10-4 | AAA_17, TM |
| 99 | - | 108943 | 110187 | 1242 | 414 | GrBNV_gp67-like | Oryctes rhinoceros virus | 29 | 9 × 10-8 | EF_HAND_1, coiled-coil |
| 100 | - | 110290 | 110595 | 303 | 101 | 11 K virion structural protein | Oryctes rhinoceros virus | 32 | 4 × 10-6 | TONB_DEPENDENT_REC_1, TM |
| 101 | - | 110705 | 111106 | 399 | 133 | |||||
| 102 | + | 111081 | 111881 | 798 | 266 | |||||
| 103 | - | 111883 | 113868 | 1983 | 661 | |||||
| 104 | + | 113631 | 113864 | 231 | 77 | |||||
| 105 | + | 114593 | 114865 | 270 | 90 | |||||
| 106 | - | 114757 | 115062 | 303 | 101 | apoptosis inhibitor IAP-1 | Buzura suppressaria NPV | 43 | 2 × 10-13 | BIR |
| 107 | - | 115114 | 115821 | 705 | 235 | HzNVORF9-like | Chelonus inanitus | 25 | 4 × 10-10 | |
| 108 | + | 115917 | 116648 | 729 | 243 | orf101 | Helicoverpa zea nudivirus 2 | 23 | 0.1 | ribokinase_pfkB_like, TM |
| 109 | - | 116750 | 116896 | 144 | 48 | |||||
| 110 | - | 116971 | 117372 | 399 | 133 | TM | ||||
| 111 | + | 117179 | 117469 | 288 | 96 | |||||
| 112 | + | 117529 | 118617 | 1086 | 362 | TM | ||||
| 113 | + | 118674 | 118928 | 252 | 84 | sortilin-related receptor precursor | Acyrthosiphon pisum | 42 | 9 × 10-9 | LDLRA_2 |
| 114 | + | 118723 | 118911 | 186 | 62 | TM | ||||
| 115 | + | 119035 | 119493 | 456 | 152 | TM | ||||