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. 2014 Aug 1;14:560. doi: 10.1186/1471-2407-14-560

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© Tringali et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Correlation between Neu5Gc-GM3 and melanoma cell malignancy. (A) HPTLC separation of gangliosides of two cell clones (2/14 and 2/21) isolated from a single patient. (B) HPTLC separation of Neu5Ac-GM3 and Neu5Gc-GM3 in NHEM-Ad, NHEM-Neo, clone 2/14, and in melanoma cell lines representative of cluster 1 (L6), cluster 2 (L34), cluster 3 (L4). (C) 14 F7 antibody immunostaining of gangliosides separated by HPTLC (NHEM-Ad, NHEM-Neo, L6 representative of cluster 1, L34 representative of cluster 2, L4 representative of cluster 3. (D) Immunohistochemical analysis of cytospin preparations of L6 representative of cluster 1 and L34 representative of cluster 2, stained with 14 F7 antibody. Original magnification, x20 (Axiovert 100). Similar results were obtained for other melanoma cell lines according to the clustering.