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. 2014 Aug 1;14:560. doi: 10.1186/1471-2407-14-560

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© Tringali et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Up-regulation of GD3 synthase and NEU3 in melanoma cells. (A) Real Time PCR analysis of the mRNA expression of GD3 synthase and (B) of NEU3 sialidase in melanoma cell lines and in melanocytes. Dotted line represents GD3 synthase and NEU3 expression level in adult melanocytes. Values are the mean ± S.D. of four independent experiments. (C) Average mRNA expression of Sp1, assessed by Real Time PCR, in melanoma cell lines and in melanocytes. Values are the mean ± S.D. of four independent experiments. (D) HPTLC separation and (E) percent quantification of melanoma cell (L6) gangliosides before and after incubation with melanoma cell membranes.