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. 2014 May;141(10):2046–2056. doi: 10.1242/dev.101519

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — The effects of ELAV removal on the expression of other Hox proteins than Ubx. (A-C) Comparison of expression levels of Abd-A protein in dissected ventral nerve cords of wild type (A) and elav⁵ mutants (B) reveals that ELAV removal decreases the overall expression of Abd-A protein within the embryonic CNS. (C) Average profile quantification of Abd-A protein along the AP axis of wild-type and elav⁵ embryos. (D-F) Expression levels of Abd-B protein are much higher in wild-type (D) than in elav⁵ mutant (E) embryos, revealing that ELAV removal exerts similar effects across all protein-coding genes within the BX-C. (F) Quantification of Abd-B protein expression in wild-type and elav⁵ mutant embryos. (G-I) The pattern and expression levels of Antp protein are unaffected by ELAV removal. Antp protein expression levels in wild-type embryos (G) are comparable to those in elav⁵ mutant embryos (H) indicating that the effects of ELAV on Hox protein expression within the CNS vary from gene to gene. (I) Quantification of Antp protein expression in wild-type and elav⁵ embryos. (C,F,I) Grey shading represents standard error. (A,B,D,E,G,H) DAPI, blue.