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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Eur J Immunol. 2008 Aug;38(8):2351–2359. doi: 10.1002/eji.200738102

Figure 1.

Figure 1

Inhibition of MTP abrogates autoreactivity of DC. (A) CD14+ monocytes were differentiated to DC in the presence of IL-4 and GM-CSF; the indicated number of DC were co-cultured with 1×105 CD1a-restricted autoreactive Mt1.50 or CD1c-restricted Ye2.3 T cells. MTPi BMS212122 (open circles) or DMSO (vehicle; filled circles) was present throughout DC differentiation and co-culture with T cells. Supernatants were assayed for IFN-γ release by ELISA. (B) Using similar experimental setup as described in part A, the tetanus toxoid-reactive, MHC class II-restricted T cell line SPF3 (1×105) was co-cultured with tetanus-toxoid loaded DC (1×105) treated with either BMS212122 or DMSO. Activation of SPF3 was analyzed by IFN-γ release into the supernatant. (C) To exclude the possibility that the presence of BMS212122 might affect T cell activation, DC (1×105) were fixed with glutaraldehyde at day 4 of differentiation from monocytes, and co-cultured with autoreactive T cell clones (1×105) restricted by CD1a (Mt1.50), CD1b (Ec2.55), and CD1c (Ye2.3). Activation was assayed by IFN-γ release into the supernatant.