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Modular and site-specific conjugation of oligonucleotides to aldehyde-tagged proteins. (A) SDS-PAGE analysis of crude reactions between aldehyde-tagged Maltose Binding Protein (MBP) and the indicated functionalized oligonucleotide (Scheme 1B). (B) MBP–DNA conjugates after purification by anion exchange chromatography. (C) MBP–DNA conjugates incubated with complementary and noncomplementary FITC–DNA and analyzed by SDS-PAGE.
