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. 2014 Jul 16;136(31):10850–10853. doi: 10.1021/ja504711n

Figure 3.

Figure 3

Modular assembly of protein-bearing DNA multimers and their interactions with live cells. (A) Assembly of protein–DNA conjugates into dimers and trimers based on oligonucleotide sequence. (B) SDS-PAGE of DNA-linked monomer (i), along with crude dimer (ii) and trimer (iii) assemblies. (C) Flow cytometry analysis of uPAR-expressing H1299 carcinoma cells incubated with DNA-scaffolded trimer bearing Fab and Alexa488-labeled MBP. (D) A field of negative-stained DNA-scaffolded MBP trimers imaged by transmission electron microscopy. (E) Fab–MBP–MBP trimers. The red arrow points to the Fab of the heterotrimeric construct. (F) SDS-PAGE of (1) MBP–DNA conjugate, (2) Fab–DNA conjugate, (3) a Fab and MBP bearing heterodimer, and (4) a Fab and MBP bearing heterotetramer using scaffolding strands with a variable length of X base pairs (here, x=16).