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. 2014 Jul 16;136(31):10850–10853. doi: 10.1021/ja504711n

Scheme 1. Modular strategies for controlling antibody scaffold geometry using DNA–conjugates of aldehyde-tagged proteins. (A) (i) At least three unique functions can be incorporated on a Y-type (e.g. IgG) antibody scaffold. (ii) Protein–DNA conjugates can be used to selectively assemble Fab-bearing trimers. (iii) The DNA may be used as a handle for assembly of more complex scaffold architectures. (B) Attachment of oligonucleotides to aldehyde tags at protein termini or internal loops may proceed through at least four strategies: direct conjugation to DMT-protected aminooxy-modified DNA 1 or HIPS-modified DNA 2; or indirect conjugation through bifunctional polyethylene glycol 3 and subsequent copper-catalyzed triazole formation with hexynyl-modified DNA 4, or copper-free triazole formation to DBCO-modified DNA 5.

Scheme 1