Extended Data Figure 3. Impaired CD1d- but not MHC-class I-restricted antigen presentation in Mttp^ΔIEC mice.

a, CD1d-mediated presentation of the exogenous lipid antigen α-GalCer to the invariant NKT-cell hybridomas 24.7 and DN32.D3 and of endogenous lipid antigens (autoreactivity) to the non-invariant NKT-cell hybridoma 14S.6 by IECs from CD1d-knockout (CD1d-KO) mice, Mttp^ΔIEC mice and wild-type littermates (Mttp^+/+) (n = 5 mice per group). b, Presentation of H2-K^b-restricted SIINFEKL by the indicated IECs (see a) to the SIINFEKL-responsive hybridoma RF33.70. c, Representative histograms of CD1d cell surface expression as determined by flow cytometry of colonic IECs of the indicated mouse strains before (left, n = 5 mice per group) and 6 h after (right, n = 5 mice per group) rectal oxazolone challenge. d, Representative haematoxylin and eosin stainings of the indicated mouse strains upon rectal challenge with oxazolone (Ox.) or vehicle (ethanol (EtOH)). Scale bar, 40 μm. Results representative of three independent experiments are shown. Mean ±s.e.m. of triplicate cultures are shown. Student’s t-test was applied.