Table 1.

List of mammary epithelial cell digestion and mammary gland lysis buffer reagents used in the adipose depletion method.

MEC digestion buffer	Final
DMEM/F12 medium (Sigma D6421)	20 mL
Trypsin (Gibco 840-7250)	30 mg
Collagenase A (Boehringer 1088793)	60 mg
Sodium fluoride (Aldrich 450022)	50 mM
Sodium orthovanadate (Aldrich 450243)	1 mM
MG lysis buffer	Final
Tris pH 7.4	50 mM
Sodium chloride	150 mM
EDTA	2 mM
Triton X-100	1%
Deoxycholate	1%
SDS	0.1%
Sodium fluoride (Aldrich 450022)	50 mM
Sodium orthovanadate (Aldrich 450243)	1 mM
Phenylmethanesulfonyl fluoride (Sigma P7626)	0.57 mM
EDTA-free Protease Inhibitor cocktail (Roche)	20 μL/mL