Optimization and characterization of hSCP for single-cell manipulation.
(a) Numerical simulation of flow profile around the hook. (b) Model
of single-cell capture by hook. Wb, width
of bypass path; Lb, length of bypass path; D, diameter of cell. Single-cell capture efficiency under
various fluid-resistance ratios (Rc/Rb) (c) as well as aspiration times and cell
concentrations (d). Rc, fluid resistance
of capture path; Rb, fluid resistance
of bypass path. (e) Single-cell droplet volume evaluation. The average
value (343 nL) is indicated by the red dotted line. (f) Quantitative
single-cell isolation including 2, 3, 5, and 10 cells. The single
cells are indicated by white dotted circles. Cells are SK-BR-3 (b–d)
and MDA-MB-231/GFP (e–f). Scale bar, 200 μm.