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. 2014 Jul 18;136(31):10858–10861. doi: 10.1021/ja5053279

Figure 4.

Figure 4

Design and mechanism of the integrated single-cell pipet (iSCP). (a) A rounded PDMS slab containing 50-plex iSCP tips was placed on a commercially available polystyrene Petri dish; red dye was injected to aid visualization. (b) Work flow of iSCP for selective isolation of single suspended and adherent cells. (c) Sequential images of single adherent cell release. After 3 h in culture, the single MDA-MB-231 cell in the 32nd iSCP tip displayed obvious adherent morphology and was then released by loading trypsin solution into the port connected to the bypass microchannel. (d) A plot of the lengths of 50 MDA-MB-231/GFP cells after 3 h in culture within the 50-plex iSCP-tips. The minimum cell length is 27 μm, and the maximum cell length is 165 μm. Average cell length (83 μm) is indicated by the red dotted line.