Table 3.
Stability rankings obtained with the different reference gene determination methods
| Rank | Comparative Delta-Ct | BestKeeper (SD) | BestKeeper (CV%) | NormFinder | GeNorm |
|---|---|---|---|---|---|
| 1 | UBQ (1.267) | UBQ (1.12) | UBQ (4.96) | RPS4 (0.613) | UBQ/RPS4 (0.952) |
| 2 | RPS4 (1.278) | ACTB (1.21) | ACTB (6.16) | UBQ (0.713) | |
| 3 | RPL17 (1.323) | RPL17 (1.46) | RPL17 (6.34) | RPL17 (0.721) | RPL17 (1.154) |
| 4 | ACTB (1.381) | RPS4 (1.65) | RPS4 (6.43) | ACTB (0.785) | ACTB (1.202) |
| 5 | GAPDH (1.431) | GAPDH (1.66) | GAPDH (6.66) | GAPDH (0.85) | GAPDH (1.290) |
| 6 | B2M (1.52) | B2M (1.70) | B2M (7.43) | B2M (0.851) | B2M (1.367) |
Stability values obtained by each method are shown in parenthesis for each candidate reference gene. Both Standard deviation (SD) and Coefficient of variation (CV) rankings are shown for BestKeeper. The genes are ranked from most stable (1) to least stable (6).