Fig. 4.

Cross sections through the muscle coats (adjusted to the plane of the myenteric plexus, asterisks) immunohistochemically quadruple stained for synaptophysin (SYN, red), α-smooth muscle actin (SMA, blue), c-kit (green), and S100 (yellow). a control specimen, b chagasic/megacolonic specimen. Note in (b) in contrast to (a): the increased thickness of both muscle layers (blue), the loosened architecture of the muscle tissue (blue), and the decreased density of the other three markers (for details see Jabari et al. 2013)