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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: J Biol Chem. 1997 Mar 7;272(10):6114–6118. doi: 10.1074/jbc.272.10.6114

Table III. Catalytic activities of wild-type and mutant eNOS.

L-Citrulline formation, cytochrome c reduction, and NADPH oxidase activity were measured at 37 °C as described under “Experimental Procedures.” Data are expressed as the mean ± S.D. for three different experiments.

L-Citrulline formation Cytochrome c reduction
NADPH oxidase activity
Without Ca2+/CaM With Ca2+/CaM Without Arg With Arg

nmol/min/mg nmol/min/mg nmol/min/mg
Wild type 106 ± 8 177 ± 14 1656 ± 91 71 ± 4 140 ± 13
E361L UDa 164 ± 10 1529 ± 73 92 ± 6 98 ± 9
E361Q UDa 178 ± 13 1568 ± 106 109 ± 11 108 ± 9
a

UD, undetectable.