Table III. Catalytic activities of wild-type and mutant eNOS.
L-Citrulline formation, cytochrome c reduction, and NADPH oxidase activity were measured at 37 °C as described under “Experimental Procedures.” Data are expressed as the mean ± S.D. for three different experiments.
| L-Citrulline formation | Cytochrome c reduction |
NADPH oxidase activity |
|||
|---|---|---|---|---|---|
| Without Ca2+/CaM | With Ca2+/CaM | Without Arg | With Arg | ||
|
| |||||
| nmol/min/mg | nmol/min/mg | nmol/min/mg | |||
| Wild type | 106 ± 8 | 177 ± 14 | 1656 ± 91 | 71 ± 4 | 140 ± 13 |
| E361L | UDa | 164 ± 10 | 1529 ± 73 | 92 ± 6 | 98 ± 9 |
| E361Q | UDa | 178 ± 13 | 1568 ± 106 | 109 ± 11 | 108 ± 9 |
UD, undetectable.