Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Inorg Biochem. 2014 Jun 27;139:93–105. doi: 10.1016/j.jinorgbio.2014.06.011

Fig. 2. Optical absorbance spectra and kinetics of the heme species observed during single turnover reaction of iNOSox (+BH4, −L-Arg) with oxygen.

Fig. 2

The reaction was identical to that described in Fig. 1 except that L-Arg was not added.

A) UV-VIS spectra of starting ferrous Fe(II) (solid line), oxy-ferrous intermediate Fe(II)O2 (dashed line) and final ferric Fe(III) (dotted line) species resolved by global analysis.

Inset: Original rapid-scan diode-array data.

B) Kinetics of Fe(II) (solid line), Fe(II)O2 (dashed line) and Fe(III) (dotted line) obtained from the global analysis. Data are expressed as percentage of the total heme concentration.