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. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: J Inorg Biochem. 2014 Jun 27;139:93–105. doi: 10.1016/j.jinorgbio.2014.06.011

Table 2.

EPR parameters for different oxygen-induced radical intermediates in eNOS_ox [46], nNOS_ox [56] and iNOS_ox.

		+BH₄, +L-arg	−BH₄, −L-arg	−BH₄, +L-arg	+BH₄, −L-arg
*eNOS_ox^	radical species	BH₄^+·	O₂^−·	O₂^−·	new radical
	g value	2.002	2.078^a, 2.006^b	2.078^a, 2.006^b	2.005^b, 1.99^c
	P_1/2 (mW)	13.8 ± 1.0	54 ± 3	51 ± 2	4.0 ± 1.5

nNOS_ox -DTT	radical species	BH₄^+·	O₂^−·	O₂^−· and new 38G	BH₄^+· and O₂^−·
	g value	2.002	2.077^a, 2.005^b	2.077^a, 1.99	2.076^a and 2.005
	P_1/2 (mW)	27.0 ± 2.7	100.0 ± 4.7	100 and 10 ± 1	26 ± 7 and 12 ± 2

nNOS_ox +DTT	radical species	BH₄^+·	O₂^−·	O₂^−· and new 38G	wide 75 G and 20% O₂^−·
	g value	2.002	2.077^a, 2.005^b	2.077^a, 1.99	2.003^b, 1.97^c
	P_1/2(mW)	27.0 ± 2.7	100.0 ± 4.7	100 and 10 ±1	37 ± 5 and 14.7

iNOS_ox^*	radical species	BH₄^+·	O₂^−·	O₂^−·	BH₄⁺ and O₂^−·
	g value	2.002	2.080^a, 2.006^b	2.080^a, 2.006^b	2.076^a and 2.005
	P_1/2 (mW)	26 ± 1	155 ± 7	126 ± 14	37 ± 4

^a

Broad peak.

^b

Crossover.

^c

Satellite signal.

^*

No Difference between samples with or without DTT. Standard error, n = 3