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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Inorg Biochem. 2014 Jun 27;139:93–105. doi: 10.1016/j.jinorgbio.2014.06.011

Table. 3.

Comparison of rate constants from single-turnover reactions catalyzed by three NOSox isoforms obtained by stopped-flow and RFQ EPR.

nNOSox(a) iNOSox(b) eNOSox(c)
Fe(II)-O2 formation >200 106 ± 7 207
Fe(II)-O2 disappearance 34.9 18.0 ± 3.7 15
BH4 radical formation 67.0 ± 10.5 20.0 ± 2.5 13.1 ± 2.7
BH4 radical decay 0.4 0.8 ± 0.2 2.0

Anaerobic ferrous NOSox solutions that contained both L-arginine and BH4 were rapid mixed with air saturated buffers at 22 °C.

(a)

Data from [56] in the presence of DTT,

(b)

data from Table 1,

(c)

data from [46].