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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Anat Rec (Hoboken). 2010 Jun;293(6):938–946. doi: 10.1002/ar.20805

FIGURE 3.

FIGURE 3

FoxA1 protein expression is affected by heparin. FoxA1 protein in whole cell lysates of isolated human AT2 cells was analyzed by Western blot. Levels of FoxA1 steadily increased from day 2 in untreated cells, as confirmed in the densitometric tracings (gray bars). FoxA1 was highly increased by heparin treatment (black bars) at days 2, 3, and 4 but was decreased on days 7 and 9 by heparin. GAPDH of the same blot is shown as a loading control. For normalization of FoxA1 densitometry, equal areas of GAPDH signal in each lane were quantified to avoid signal overlap.