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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Anat Rec (Hoboken). 2010 Jun;293(6):938–946. doi: 10.1002/ar.20805

FIGURE 5.

FIGURE 5

Wnt7A expression in isolated human alveolar type 2 cells varies with time and heparin treatments. Quantitative real time polymerase chain reaction (qRT-PCR) using TaqMan primers and probes was performed on cDNA equivalent to 100 ng of total RNA isolated from human AT2 cells cultured with or without 500 μg/mL heparin for 0–9 days. The absolute wnt7A gene regulation in cells from three individuals treated in separate experiments with (▲) or without (●) heparin is depicted at each time point. The solid line (—) connects the means of the expression at each time point in heparin-treated cells from the three individuals while the broken line (---) connects the means of expression at each time point in untreated cells. Wnt7A clearly increased rapidly from day 2 through day 9 and was enhanced by heparin on days 7 and 9.