Table 1.
Primers (positiona) | Sequences |
---|---|
17-mer (−4) | 5'-AACGACGGCCAGTGAAT-3' |
18-mer (−3) | 5'-AACGACGGCCAGTGAATT-3' |
19-mer (−2) | 5'-AACGACGGCCAGTGAATTC-3' |
20-mer (−1) | 5'-AACGACGGCCAGTGAATTCG-3' |
21-mer (0) | 5'-AACGACGGCCAGTGAATTCGC-3' |
22-mer (+1) | 5'-AACGACGGCCAGTGAATTCGCG-3' |
Templates | |
26-mer | 3'-TTGCTGCCGGTCACTTAAGCGCGCCC-5' |
b26-mer-dGC8-N-ABA | 3'-TTGCTGCCGGTCACTTAAGCGCGCCC-5' |
DNA trap | |
D-1 (21/41-mer) | 5'-CGCAGCCGTCCAACCAACTCA-3' |
3'-GCGTCGGCAGGTTGGTTGAGTAGCAGCTAGGTTACGGCAGG-5' |
Position of primer 3′-terminus relative to the DNA lesion dGC8-N-ABA.
G designates dGC8-N-ABA.