Table 3.

Kinetic parameters for dNTP incorporation into damaged DNA substrates containing dG^C8-N-ABA

dNTP	Kd,dNTP (µM)	kp (s−1)	(kp/Kd, dNTP)damaged (µM−1s−1)	Efficiency Ratioa,b	Fidelityc	Probabilityd
18/26-mer-dGC8-N-ABA
dCTP	32 ± 5	2.83 ± 0.08	0.088	1.25	-	99.95
dATP	2200 ± 600	(2.0 ± 0.4) × 10−2	9.1 × 10−6	0.85	7.3 × 10−6	0.01
dGTP	350 ± 40	(6.2 ± 0.3) × 10−4	1.8 × 10−5	1.6	1.4 × 10−5	0.02
dTTP	-	-	1.6 × 10−5	5.4	1.3 × 10−5	0.02
19/26-mer-dGC8-N-ABA
dGTP	50 ± 9	0.48 ± 0.02	9.6 × 10−3	2.6	-	99.82
dATP	430 ± 60	(2.9 ± 0.2) × 10−3	6.7 × 10−6	2.1	7.0 × 10−4	0.07
dCTP	220 ± 80	(1.4 ± 0.2) × 10−3	6.4 × 10−6	22	6.7 × 10−4	0.06
dTTP	-	-	5.5 × 10−6	1.6	5.7 × 10−4	0.05
*20/26-mer-dGC8-N-ABA
dCTP	510 ± 60	0.25 ± 0.02	4.9 × 10−4	120	-	98.93
dATP	640 ± 170	(1.9 ± 0.3) × 10−4	3.0 × 10−6	6.3	6.1 × 10−4	0.61
dGTP	1200 ± 400	(8.1 ± 0.1) × 10−4	6.8 × 10−7	66	1.4 × 10−4	0.14
dTTP	970 ± 390	(1.6 ± 0.4) × 10−4	1.6 × 10−6	52	3.3 × 10−4	0.32
*21/26-mer-dGC8-N-ABA
dGTP	400 ± 80	(8.9 ± 0.7) × 10−4	2.2 × 10−5	170	-	84.26
dATP	450 ± 50	(4.5 ± 0.2) × 10−4	1.0 × 10−6	2.4	4.3 × 10−2	3.83
dCTP	160 ± 20	(4.3 ± 0.1) × 10−4	2.7 × 10−6	12	1.1 × 10−1	10.34
dTTP	420 ± 80	(1.7 ± 0.1) × 10−4	4.0 × 10−7	4.5	1.8 × 10−2	1.57
22/26-mer-dGC8-N-ABA
dCTP	1400 ± 300	4.5 ± 0.7	3.2 × 10−4	11	-	99.55
dATP	470 ± 70	(5.4 ± 0.4) × 10−4	1.1 × 10−5	0.38	3.4 × 10−4	0.34
dGTP	1100 ± 200	(1.4 ± 0.2) × 10−4	1.3 × 10−6	6.5	4.1 × 10−4	0.04
dTTP	770 ± 140	(1.7 ± 0.2) × 10−4	2.2 × 10−6	5.0	6.9 × 10−4	0.07

^*Denote pause sites.

^aCalculated as (k_p/K_{d, dNTP})_control/(k_p/K_{d, dNTP})_damaged.

^bValues for (k_p/K_{d, dNTP})_control 19/26-mer to 22/26-mer are from supplemental Table 1 of reference [10]. Kinetic parameters for 18/26-mer are listed in SI Table 1.

^cCalculated as (k_p/K_{d, incorrect dNTP)damaged}/((k_p/K_{d, correct dNTP})_damaged + (k_p/K_{d, incorrect dNTP})_damaged).

^dCalculated as ((k_p/K_{d, dNTP})_damaged/(Σ(k_p/K_{d, dNTP})_damaged)) × 100.