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Photon counting SHG imaging shows increased reproducibility. Stability comparisons between analog (■) and digital (●) detections. Each solid point represents data from one voltage-clamp experiment. □ (○) shows mean value with standard error from analog (digital) detections. For analog detection, the average SHG change is 9.36 ± 0.64 (mean ± SE), while the number is 11.73 ±0.52 for detection using photon counter.
