Table 4.

Results of statistics for the effect of acute methamphetamine challenge on mouse behavior represented in Table 3. Two-way ANOVA was applied to all behavior categories except the “Running wheel” category, which was analyzed by one-way ANOVA. The F and P values are shown in upper and lower columns, respectively.

	Factor-Total (09:30-09:00)			Initial 3 h (09:30–12:30)			Dark period (19:00-07:00)
Behavior category	Exposure to wheel (E) F value P value	Challenge (C)	E × C	E	C	E × C	E	C	E × C
Running wheel	N/A	2.590	N/A	N/A	0.073	N/A	N/A	4.549	N/A
	N/A	0.1249	N/A	N/A	0.7907	N/A	N/A	< 0.05	N/A
Locomotion	21.572	0.008	3.179	5.169	17.870	1.643	29.931	8.283	2.982
	< 0.0001	0.9288	0.0830	< 0.05	< 0.001	0.2081	< 0.001	< 0.01	0.0928
Food access	18.755	12.174	0.129	3.471	11.022	1.693	16.269	0.263	1.211
	< 0.0001	< 0.01	0.7213	0.0706	< 0.01	0.2015	< 0.001	0.6113	0.2784
Food intake	0.642	2.323	2.933	13.384	13.384	2.108	3.201	0.836	0.405
	0.4282	0.1362	0.0954	< 0.001	< 0.001	0.1552	0.0820	0.3666	0.5287
Water intake	6.506	1.269	0.014	2.905	0.984	0.016	4.527	0.123	0.011
	< 0.05	0.2674	0.9077	0.0969	0.3278	0.8995	< 0.05	0.7275	0.9183