Table 2.
GlcNAc-transferase activities that synthesize N-glycan antennae. Assays were carried out by HPLC as described in Material and Methods. The results were calculated as the average of duplicates that differ by<10 %. Abbreviations are as in Table 1. Caco-2 cells served as positive controls. RWPE-1 cells were derived from normal prostate. PC-3, DU145, LNCaP and VCaP cells are metastatic prostate cancer cells
| Enzyme | Activity (nmol/h/mg)
|
|||||
|---|---|---|---|---|---|---|
| RWPE-1 | PC-3 | DU145 | LNCaP | VCaP | Caco-2 | |
| GnT-I | 7.21 | 1.65 | 1.10 | 3.94 | 2.60 | 3.45 |
| GnT-II | 3.40 | 1.19 | 1.55 | 5.64 | 2.79 | 2.44 |
| GnT-V | 0.03 | 0.31 | 0.05 | 0.35 | 0.23 | 0.08 |