Table 4.
Activities of extension and termination glycosyltransferases and sulfotransferases. Assays were carried out by HPLC as described in Material and methods. Abbreviations are as in Table 1 and 2
| Enzyme | Activity (nmol/h/mg)
|
|||||
|---|---|---|---|---|---|---|
| RWPE-1 | PC-3 | DU145 | LNCaP | VCaP | Positive control | |
| Extension β3GlcNAcT | 0.1 | 0.3 | 0.1 | 0.3 | < 0.1 | 1.1b |
| Elongation β3GlcNAcT | 0.1 | 0.2 | 0.1 | 0.1 | 0.1 | 1.4b |
| β3/4GalNAcT (Gnβ3GalNAcα-pnp substrate) | < 0.1 | < 0.1 | 0.2 | 0.4 | 0.2 | 15.3b |
| β3/4GalNAcT (Gnβ-Bn substrate) | < 0.1 | < 0.1 | < 0.1 | 0.1 | < 0.1 | 0.2b |
| β3/4GalT (Gnβ-Bn substrate) | 25.9 | 9.3 | 31.7 | 39.7 | 17.2 | 38.4a |
| β3/4GalT (Gnβ3GalNAcα-pnp substrate) | 6.3 | 3.7 | 6.9 | 13.3 | 8.4 | 13.1a |
| α2FUT1 | 0.9 | < 0.1 | 0.2 | 0.6 | 0.7 | >10b |
| α2FUT2 | 0.8 | 0.2 | 0.8 | 0.3 | 0.7 | >10b |
| α3FUT | 0.6 | 0.2 | 0.2 | 0.2 | 0.3 | 3.1b |
| α4FUT3 | 1.1 | < 0.1 | < 0.1 | < 0.1 | 0.1 | 0.8b |
| ST3Gal | 0.4 | 17.3 | 0.2 | 7.7 | 1.3 | 7.0c |
| Activity (pmol/h/mg) | ||||||
| Gal3ST | 3.2 | 0.5 | 0.7 | 0.6 | 1.6 | 148.7b |
| GlcNAc6ST | 5.3 | 0.2 | 0.1 | < 0.1 | < 0.1 | 0.5b |
a
Enzyme activity in Caco-2 cell homogenates
b
activity in rat colon mucosal homogenates
c
ST3Gal activity in mouse intestine homogenates from ST3Gal1 overexpressing mice [61]