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. 2014 Aug 14;10(8):e1004521. doi: 10.1371/journal.pgen.1004521

Figure 4. EVA-1 interacts with UNC-40 via its transmembrane domain.

Figure 4

A, A′ and A″. Shown is a single muscle cell that expresses EVA-1::CFP (expressed from an extra-chromosomal array) (A) that is enriched at muscle arm termini (arrowhead), and functional UNC-40::YFP (expressed from the trIs34 array) (A′), and their co-localization (A″). The white arrow in the left-hand panels points to an example of muscle arm termini. The area that is boxed in each left-hand panel is respectively magnified in each right-hand panel. The arrow in the right-hand panels indicates one area of co-localization. The lack of dependence of EVA-1 and UNC-40 localization on the presence of UNC-40 and EVA-1, respectively, is shown in Figure S3a and S3b. The scale bar represents 50 µM. B. A western blot showing that UNC-40 co-immunoprecipitates with full-length FLAG-tagged EVA-1, but not with a version of EVA-1 whose transmembrane domain has been swapped for that of PAT-2. Control blots showing a lack of interaction between EVA-1 and PAT-2 and between UNC-40 and PAT-2 are shown in Figure S3c and S3d.