Figure 7. EP4 silencing decreased the expression of enzymes involved in the TGF-β1-induced synthesis of PGE2.

A, B, C: The AD-Cre and AD-GFP infected EP4^Flox/Flox mice GMCs were stimulated by 10 ng/ml TGF-β1 for 24 h. The expressions of COX1, COX2, mPGES-1, mPGES-2 and cPGE were assessed by Western Blot. (A: Western Blot; B, C: Quantification of COX1, COX2, mPGES-1, mPGES-2 and cPGE expression is achieved using densitometric values normalized to β-actin levels); D, E: The expressions of COX1, COX2, mPGES-1, mPGES-2 and cPGE were assessed by RT-PCR. (*P<0.05, **P<0.01 vs Control group, the means and error bars are the result of biological replicates).