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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 2;1823(8):1316–1323. doi: 10.1016/j.bbamcr.2012.05.025

Fig. 1. TP activity is necessary for its inhibitory effect on VSMC proliferation.

Fig. 1

A. Phagemid vector encoding human wild type TP or K115E (Lys-115-->Glu), L148R (Leu-148-->Arg) and R202S (Arg-202-->Ser) mutant TP were reversely transfected into 105 mouse primary cultured VSMC in 6-well plate using Lipofectamine LTX Plus Regent. The cell numbers were counted 72 hrs later with Z™ Series COULTER COUNTER®. N=3 for each transfection. *p<0.01, pBR-RSV/TP vs. others. B. 104 primary cultured mouse VSMC was seeded in 12-well plate, cultured for overnight and then subjected for serum starvation for 24 hrs. The cells were stimulated with 1% FCS in the presence C2 or PC cell lysate for 24 hrs and the cell proliferation was assessed with MTT assay. N=4 for each condition.