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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 2;1823(8):1316–1323. doi: 10.1016/j.bbamcr.2012.05.025

Fig. 5. Unphosphorylated STAT3 inhibits VSMC proliferation.

Fig. 5

A. Mouse wt-stat3 and Y705F mutant stat3 were transfected into PC cells and transfected gene overexpressing cell clones were generated by blasticidin (10 µg/ml) and G418 (1 mg/ml) double selection. Whole cell lysates were subjected for Western blot assay using antibody to total STAT3. Actin was re-blotted as loading control. B. 104 cells were planted into 6-well plate, and cultured under normal conditions. The cell numbers were counted daily up to 3 days, n=3. * p<0.01, PC vs. others at the same day. C. 104 cells were planted into 12-well plates and cultured under normal conditions. Culture media was changed every 2 days. Cell proliferation was assessed by MTT assay at the indicated days. Data were represented as fold change of Day 0, which MTT assay was performed 6 hrs later when the cells completely adhered to the plate. n=3. * p<0.01, PC vs. others at the same day. D. 1.5 × 105 mouse primary cultured VSMCs were planted into 6-well plate and transfected with indicated plasmid vectors. Normal cultured parent VSMC (No transfection) was used as control. The cell numbers was analyzed with Z™ Series COULTER COUNTER®. N=3.