Figure 6. LAG-3-deficient mice produce higher amounts of cytokine following mercury treatment.

Wild-type and Lag-3^−/− mice were either given a challenge dose of HgCl₂ (30 µg/mouse s.c. on days 0, 2 and 4) or were left untreated. Splenocytes were harvested on day 8 and then stimulated in vitro with plate bound anti-CD3 and anti-CD28 antibodies. Supernatant were collected after 72 hrs to determine the levels of cytokine by ELISA (n = 3 or 4). Two way ANOVA was used to determine the statistical significance between the groups where *indicates p<0.05 and ***indicates p<0.0001.