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. 2014 Aug 14;9(8):e103122. doi: 10.1371/journal.pone.0103122

Figure 1. MβCD reduced cholesterol dose-wise in human peripheral blood eosinophils, while MβCD+Chol (2% cholesterol) elevated cholesterol.

Figure 1

Three to five-hundred thousand eosinophils per treatment were treated 1 hour with media, MβCD, or MβCD+2%Chol (A–C) then fixed in 2% paraformaldehyde, stained with 50 µg/mL Filipin III (cholesterol stain), and analyzed via flow cytometry. (A) Pooled data from dose-wise treatment (n = 5). (B) Representative histogram from n = 16 experiments of cells stained with FIII after treated with i. media, ii. 5 mg/mL MβCD, iii. or 5 mg/mL MβCD+2%Chol, as well as unstained eosinophils (iv.). (C) Quantification of 16 independent experiments of FIII-stained eosinophils after treatment with 5 mg/mL MβCD or 5 mg/mL MβCD+2%Chol. Unmarked comparisons were non-significant.